Merck, 10736988001, Creatine Kinase (CK)

Name: Creatine Kinase (CK)
Brand: Merck
SKU: 10736988001

Size:500 mg, creatine phosphokinase, from rabbit muscle

Catalog Number: 10736988001

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Category

Modifying Enzymes

Pack Size500 mg

TagsLabware Labware Partners Roche<sup>®</sup> Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.
Application
Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.
Biochem/physiol Actions
Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.
Features and Benefits
Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.
Physical form
Lyophilizate, stabilized
Preparation Note
Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.

Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Analysis Note
Contaminants: <0.001% ATPase, HK, and myokinase, each
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.

Specifications

General

UNSPSC Code	12352204
Enzyme Commission (EC) Number	2.7.3.2 ( BRENDA \| IUBMB )
NACRES	NA.54
biological source	rabbit muscle
form	lyophilized
packaging	pkg of 100 mg (10127566001)
manufacturer/tradename	Roche
color	white to light yellow
optimum pH	6.0-7.0(reverse reaction)
solubility	water: soluble
suitability	suitable for immunoprecipitation
NCBI accession no.	NP_001075708
UniProt accession no.	P00563
application(s)	life science and biopharmasample preparation
foreign activity	ATPase <0.001%
storage temp.	2-8°C
Gene Information	rabbit ... CKM(100009056)

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Merck, 10736988001, Creatine Kinase (CK)

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