Merck, 10791156001, Endoproteinase Glu-C (V8 Protease)

Name: Endoproteinase Glu-C (V8 Protease)
Brand: Merck
SKU: 10791156001

Size:2 mg, V8 protease, protease v8, from Staphylococcus aureus V8

Catalog Number: 10791156001

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Category

Protease Inhibitors

Pack Size2 mg

TagsLabware Labware Partners Roche<sup>®</sup> Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).
At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.
Application
Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.
Biochem/physiol Actions
Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.
Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
Preparation Note
Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Store at 2 to 8 °C. (Store dry!)
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

Enzyme Commission (EC) Number	3.4.21.19 ( BRENDA \| IUBMB )
UNSPSC Code	23201100
NACRES	NA.54
form	lyophilized (salt-free)
specific activity	20 U/mg
mol wt	30 kDa
packaging	pkg of 2 mg
manufacturer/tradename	Roche
optimum pH	8.0-8.5

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