Merck, 1160100001, Superspher® 60 RP-8 LiChroCART™ 250-4

Supelco Superspher 60 RP-8 LiChroCART is a reversed-phase C8 chromatography cartridge packed with 4 µm spherical silica particles and a 60 Å pore size. The analytical cartridge measures 250 mm in length and 4 mm in internal diameter. Octyl surface chemistry is suitable for separating a range of neutral, acidic and weakly basic compounds under appropriately selected HPLC conditions. The packing is not endcapped, which can influence retention and peak shape compared with endcapped C8 phases. The LiChroCART design must be installed in a compatible manuCART holder before use. After fitting the cartridge, equilibrate with the intended mobile phase and establish baseline retention and pressure before analyzing samples. The listed operating pH range is 2–7.5; mobile-phase changes outside the allowable conditions can shorten column life. Maintain consistent sample preparation and filtration practices to limit particulate loading. The RP-8 configuration is distinct from the RP-select B version even though their nominal dimensions are similar.