Merck, N7285-100ML, Ninhydrin Reagent
Size:100 mL, 1, 2, 3-Indantrione monohydrate, 2, 2-dihydroxyindene-1, 3-dione, Ninhydrine, 2% Solution
Catalog Number: N7285-100ML
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Category
Pack Size100 mL
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| UNSPSC Code | 12352202 |
|---|---|
| EC Number | 210-123-3 |
| NACRES | NA.56 |
| form | liquid |
| storage temp. | 2-8°C |
| Quality Level | 200 |
Answer:The product is supplied as a 2% ninhydrin solution in a 100 mL bottle.
Answer:It is used for solution-based colorimetric determination of amino acids and suitable amine-containing compounds.
Answer:Store at 2–8 °C, protect from light and minimize air exposure to prevent oxidation.
Sigma-Aldrich Ninhydrin Reagent is a ready-prepared 2% solution supplied in a 100 mL bottle for colorimetric determination of amino acids and other suitable amine-containing compounds. The formulation contains ninhydrin and hydrindantin in dimethyl sulfoxide and a lithium acetate buffer system near pH 5.2. Reaction with amino acids produces colored products that can be measured spectrophotometrically under an established procedure. For a quantitative assay, prepare calibration standards, maintain consistent heating and cooling conditions and evaluate interfering sample components. The reagent is sensitive to air; progressive oxidation can alter its original burgundy color and impair performance. Protect it from light, reduce unnecessary exposure to air and store at 2–8 °C. Use appropriate chemical protection when handling the solvent-containing solution. This particular formulation is intended for solution-based determination and should not be substituted automatically for a TLC spray or dipping reagent. Confirm assay conditions and analytical acceptance criteria with appropriate controls before comparing sample results.
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