Merck, 1161220500, Tryptone Bile X-glucuronide agar
Size:500 g, TBX agar, Tryptone Bile Glucuronide medium, ChromoCult®, ISO 16649, granular, for Escherichia coli, pkg of 500 g
Catalog Number: 1161220500
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Pack Size500 g
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| NACRES | NA.74 |
|---|---|
| UNSPSC Code | 41106213 |
| agency | ISO 16649 |
| form | granular |
| packaging | pkg of 500 g |
| manufacturer/tradename | ChromoCult® |
| technique(s) | microbiological culture: suitable |
| pH | 7.0-7.4 (25 °C, 36.6 g/L in H2O, after autoclaving) |
| solubility | 31.6 g/L |
| application(s) | food and beveragesmicrobiology |
| storage temp. | 15-25°C |
| suitability | Escherichia coli |
| Quality Level | 200 |
Answer:It is designed for detection and enumeration of β-glucuronidase-positive Escherichia coli.
Answer:The medium is associated with ISO 16649 methodology.
Answer:Typical β-glucuronidase-positive E. coli colonies develop a turquoise-to-blue appearance.
Tryptone Bile X-glucuronide agar, ChromoCult® is used for detection and enumeration of β-glucuronidase-positive Escherichia coli in food, feed, and environmental samples. The granular dehydrated medium follows ISO 16649 methodology and combines tryptone, bile components, the chromogenic X-glucuronide substrate, and agar to provide selective and differential growth. E. coli strains with β-glucuronidase activity form characteristic turquoise-to-blue colonies, allowing presumptive identification directly on the medium, while non-target organisms produce different colony appearances or are inhibited. The prepared medium has a specified pH range of 7.0–7.4 at 25°C after autoclaving. Quality-control testing includes recovery and selectivity checks against defined microbial strains. The 500 g pack is suitable for laboratories performing food microbiology, hygiene monitoring, and environmental microbiological testing where standardized E. coli enumeration is required.
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