Merck, 16670-100MG, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Brand: Merck
SKU: 16670-100MG

Size:100 mg, BCIP® p-toluidine salt, X-phosphate p-toluidine salt, ≥95.0% (HPLC)

Available Catalog Numbers

16670-100MG 16670-1G

Catalog Number: 16670-100MG

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Category

ELISA Substrates

Pack Size100 mg

TagsProtein Biology Proteins & Enzymes Detection Substrates & Enzymes

CollectionsMerck Proteins & Enzymes: Nucleases, Albumins & High‑Purity Enzymes Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

NACRES	NA.83
PubChem Substance ID	57647586
UNSPSC Code	12352204
Web Name Suffix Override	≥95.0% (HPLC), powder
EC Number	229-506-1
MDL number	MFCD00040636
InChI	1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key	QEIFSLUFHRCVQL-UHFFFAOYSA-N
SMILES string	Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
assay	≥95.0% (HPLC)
form	powder
storage temp.	2-8°C
Quality Level	200

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