Merck, 233501-100UG, Coagulation Factor XIII, Human Plasma

General description
Native coagulation factor XIII from human plasma. Plasma Factor XIII is a tetramer (α₂β₂) composed of two identical α-subunits and two identical β-subunits. After thrombin cleavage converts the α₂β₂ tetramer to a modified α₂′β₂ tetramer, Ca²⁺ promotes both the dissociation of the β₂ dimer and the activation of the α₂′ dimer to its catalytically active form. This activated Factor XIII (Factor XIIIa) strengthens the fibrin clot by catalyzing the formation of glutamyl-lysine bonds between fibrin monomers.
Physical form
In 500 µM EDTA, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma. One unit is defined as the activity in 1 ml of normal, pooled plasma.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)