Merck, 345736, Ganglioside GD_1a, Disialo, Diammonium Salt, Bovine Brain

General description
Disialoganglioside prepared from bovine gray matter. Useful as a differentiation marker of tumor cell growth. Protein kinase C inhibitor. Readily converted to GM₁ by bacterial and mammalian sialidases without detergent or bile salts. One mole yields 2 moles of sialic acid by thiobarbituric acid assay. The molecular weight is based on stearoyl-18:0.

Note: This product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock.
Useful as a differentiation marker of tumor cell growth. Protein kinase C inhibitor. Readily converted to GM₁ by bacterial and mammalian neuraminidases without detergent or bile salts. Note: the moleculare weight is based on on stearoyl-18:0.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Protein kinase C
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
For use in aqueous systems, dissolve in a small amount of DMSO and then sonicate in buffer or medium, or dissolve the dried sample directly in aqueous buffers by sonication. Solutions may be filter sterilized.
Other Notes
Longee, D.C., et al. 1991. Acta Neuropathologica82, 45.
Livingston, P.O., et al. 1987. Proc Natl. Acad. Sci. USA84, 2911.
Kundu, S.K., and Scott, D.D. 1982. J. Chromato.232, 19.
Wakomari, S. 1981. Annu. Rev. Biochem.50, 733.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)