Merck, 431525-100U, L-Leucine Dehydrogenase, Bacillus cereus

General description
Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
Physical form
In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.
One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)