General description
cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete, Mini, contains EDTA and both irreversible and reversible protease inhibitors.
Application
cOmplete™, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete, Mini, contains EDTA (1 tablet yields a 1 mM EDTA solution in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete, Mini, EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is present.
cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml), we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore, comparable results are achieved with both product types.
cOmplete™, Mini Protease Inhibitor Cocktail has been used as a component in
mammalian-protein extraction reagent (M-PER) for lysis of glioma stem cells (GSCs)
sodium dodecyl sulphate-tris buffered saline (SDS-TBS) for the homogenisation of brain tissues
radioimmunoprecipitation assay (RIPA) buffer for lysis of human embryonic stem cells (hESCs)
Biochem/physiol Actions
For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
Features and Benefits
Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.
Packaging
30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.
Physical form
Tablets in foil blister packs
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution
Preparation of Working Solution:
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent breakage of tablets.
One cOmplete Mini tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. The tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.):
Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.
Analysis Note
Performance-tested with pancreas extract.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Legal Information
cOmplete is a trademark of Roche
