Merck, 539251-1MG, Protein A, Alkaline Phosphatase Conjugate

General description
Native protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
Native protein A from Staphylococcus aureus conjugated to alkaline phosphatase.
Application
Direct Immunoassay (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
Analysis Note
Single band by SDS-PAGE
Other Notes
p-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)