Description
General description
""I use GeneJuice because it′s ease-of-use, low toxicity and wide range of target cells""
Stuart Rulten. Research Fellow. University of Sussex
""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany
""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland
Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
Cell lines transfected with GeneJuice® Transfection ReagentCell Lines:Primary Cells:
10T1/2
293T
3T3 NIH
3T3 Swiss
3T3-L1
A204
A431
A549
alpha TC1-6
AR 42J
As4.1
AtT-20
B50
BC-1
BC-2
BC3
BCBL
BHK-21
C3H/10T1/2
C6
C2C12Caco-2
Caki-1
Calpan-1
Calu-1
Calu-6
CCL-131
CFPAC-1
Chang Liver
CHO
CHO-7
CHO-IR
CHO-K1
COS-1
COS-7
CS-1
CV-1
Daudi
DDTI MF-2
DT40
ECV304
EL4ES-E14TG2a
EVSCC17M
H9c2
HCT-116
HEK293
HeLa
HeLa B
HeLa T4
Hep 3B2.1-7
HepG2
Hepa 1-6
Ht-29
HTB-37
HTB-45
Huh-7
HUVEC
IC21
IEC-6
JEG-3
Jurkat
KBL57-3-11
L-6
L-929
MA-10
McA-RH7777
MCF-7
MCF-10-2A
MDCK
Melanocyte
MG-63
Neuro 2A
Neuroblastoma
NRK
NT2/D1
OV-1063
OVCAR3
P4
P19
PC12
PA317
PAM212PS-1
R2C
RAW 264.7
RBL-2H3
RMP-41
SAOS-2
SC-1
Schneider line2
SK-N-MC
SK-N-SH
SKOV3
STO
SW-480
SW-837
T3M4
TM4
U937
UCD
Vero
Aortic smooth muscle cells
Astrocytes
Angioblasts
Chondrocytes
Chromaffin cells
Epithelial cells:
mammary
prostate
tracheal
Fibroblasts
Keratinocytes
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
Features and Benefits
Highly efficient DNA transfer for both stable and transient transfections
Compatibility with both serum-containing and serum-free media
Simple protocolno need for media changes
Ideal for high-throughput transfection in a multi-well plate format
Ideal for retrovirus production for T-cell transduction
Works for a multitude of cell types with minimal optimization
Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers
The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates
Preparation Note
For protocol, please click (here)
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
GeneJuice® is a registered trademark of EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Flammable (J)
