Merck, 99011338-DNA-5UG, AFRC IAH-CC101

Immunogen
bovine Con-A- stimulated lymphoblasts
Application
Flow cytometry, Immunohistology, Immunoprecipitation
Biochem/physiol Actions
Mouse x Mouse hybridoma
The monoclonal antibody CC101 was produced by inoculating mice with bovine lymphocytes activated with concanavalin A (Con A) The antibody is specific for the WC1 molecule on gamma delta T lymphocytes in cattle that also reacts with porcine, sheep and goat cells. It recognizes a 180,000 MW molecule on the majority of pig CD2- CD4- CD8- T cells. Cells have been grown on macrophage feeder cells and using hypoxanthine and thymidine at concentrations appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Preparation Note
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37°C.
RPMI 1640 + 10% FBS and HT supplement
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].