Merck, B5285-100MG, 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt

Name: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt
Brand: Merck
SKU: B5285-100MG

Size:100 mg, BC-Indicator, X-GlcA, X-glucuronide, ≥98%

Available Catalog Numbers

B5285-100MG B5285-10MG B5285-25MG

Catalog Number: B5285-100MG

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Category

ELISA Substrates

Pack Size100 mg

TagsProtein Biology Proteins & Enzymes Detection Substrates & Enzymes

CollectionsMerck Proteins & Enzymes: Nucleases, Albumins & High‑Purity Enzymes Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used:
as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay
to prepare X-Gluc or GUS staining solution
to confirm GUS expression in samples by X-gluc staining

Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

Specifications

General

UNSPSC Code	12352204
PubChem Substance ID	24891832
NACRES	NA.83
Web Name Suffix Override	≥98%, powder
MDL number	MFCD00135782
SMILES string	[Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI	1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1
InChI key	IBLSVGDGSKUDCT-CYRSAHDMSA-M
assay	≥98%
form	powder
solubility	water: 50 mg/mL, clear, colorless
storage temp.	−20°C
Quality Level	200

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