| UNSPSC Code | 12171500 |
|---|---|
| Web Name Suffix Override | methanol solution |
| NACRES | NA.47 |
| PubChem Substance ID | 24892085 |
| MDL number | MFCD00041762 |
| Beilstein/REAXYS Number | 5718025 |
| Colour Index Number | 42660 |
| InChI | 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1 |
| SMILES string | [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1 |
| InChI key | NKLPQNGYXWVELD-UHFFFAOYSA-M |
| form | methanol solution |
| color | dark blue |
| suitability | suitable for SDS-PAGE |
| application(s) | diagnostic assay manufacturinghematologyhistology |
| storage temp. | room temp |
| Quality Level | 200 |
Answer:It is used to prepare protein gel stains for visualization of bands after electrophoresis.
Answer:No. It is a methanol-based concentrate; the working solution must be prepared for the chosen staining protocol.
Answer:No. It supplies Coomassie Brilliant Blue R staining dye rather than a protein ladder or prestained marker.
Sigma-Aldrich Brilliant Blue R Concentrate is a methanolic concentrate of Coomassie Brilliant Blue R dye for protein visualization following polyacrylamide gel electrophoresis. The dye binds to proteins and produces blue-stained bands that assist assessment of electrophoretic separations. This product is a staining reagent concentrate, not a prestained protein ladder, fluorescent assay kit or finished polyacrylamide gel. Preparation of a working stain and the associated destaining steps depend on the selected SDS-PAGE staining protocol. Methanol in the supplied formulation requires appropriate solvent-compatible handling and disposal. The 1-each format suits routine laboratory protein gel staining preparations.
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