Merck, B9146-100MG, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Name: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
Brand: Merck
SKU: B9146-100MG

Size:100 mg, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal, ≥98%

Available Catalog Numbers

B9146-100MG B9146-10MG B9146-500MG

Catalog Number: B9146-100MG

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Category

ELISA Substrates

Pack Size100 mg

TagsProtein Biology Proteins & Enzymes Detection Substrates & Enzymes

CollectionsMerck Proteins & Enzymes: Nucleases, Albumins & High‑Purity Enzymes Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
Preparation Note
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

Specifications

General

Web Name Suffix Override	≥98%, powder, suitable for identification of lac+ bacterial colonies
NACRES	NA.83
PubChem Substance ID	57653968
UNSPSC Code	12352204
EC Number	230-640-8
MDL number	MFCD00005666
Beilstein/REAXYS Number	1402009
InChI key	OPIFSICVWOWJMJ-AEOCFKNESA-N
InChI	1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
SMILES string	OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
assay	≥98%
form	powder
solubility	DMF: methanol (1:1): soluble 50 mg/mL
suitability	suitable for identification of lac+ bacterial colonies
storage temp.	−20°C

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