Merck, CB1024-100UG, Anti-ACTN4 Mouse mAb (4D10)

General description
Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
Immunogen
A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)