Merck, CNSBR005-1G, Carbenicillin disodium salt

General description
Carbenicillin is a semi-synthetic, broad-spectrum carboxypenicillin antibiotic with bactericidal and β-lactamase resistant activity. It is a broad-spectrum antibiotic, meaning that it is effective against a wide range of bacteria, including both Gram-positive and Gram-negative bacteria. Carbenicillin is particularly useful against Pseudomonas aeruginosa, a Gram-negative bacterium that is often resistant to other antibiotics. Carbenicillin is commonly used in cell biology applications to prevent the growth of bacterial contaminants. It is also used in microbiology to select for bacteria that have been transformed with a vector harboring the gene encoding β-lactamase, which makes them resistant to carbenicillin.
Application
Carbenicillin disodium salt has been used: in the preparation of Luria-Bertani (LB) agar plates and mediacitationx as a selective agent in the culture media to prevent the growth of bacterial contaminantscitation in a study focused on the development of monoclonal antibodiescitation
Biochem/physiol Actions
Antimicrobial spectrum: Carbenicillin acylates the penicillin-sensitive transpeptidase C-terminal domain by opening the lactam ring. This inactivation prevents the cross-linkage of peptidoglycan strands, thereby inhibiting the third and last stage of bacterial cell wall synthesis. This leads to incomplete bacterial cell wall synthesis and eventually causes cell lysis.
Mode of Action: Carboxypenicillin antibiotic that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Antimicrobial spectrum: Active against Gram-positive and Gram-negative bacteria Carbenicillin acylates the penicillin-sensitive transpeptidase C-terminal domain by opening the lactam ring. This inactivation prevents the cross-linkage of peptidoglycan strands, thereby inhibiting the third and last stage of bacterial cell wall synthesis. This leads to incomplete bacterial cell wall synthesis and eventually causes cell lysis.
Mode of Action: Carboxypenicillin antibiotic that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Antimicrobial spectrum: Active against Gram-positive and Gram-negative bacteria
Features and Benefits
High quality antibiotic suitable for mulitple research applications.
Analysis Note
Stable at 37 °C for 3 days