Merck, CS0001-1KT, Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)

Name: Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)
Brand: Merck
SKU: CS0001-1KT

Size:1 kit, DDPC, DEPC, DLOPC, DLPC, DMPC, DOPC, DPPC, DSPC, EPC, HEPC, HSPC, LYSOPC, MPPC, MSPC, PC, PMPC, POPC, PSPC, Phosphatidylcholine, SMPC, SOPC, lecithin, lysolecithin, sphingomyelin, sufficient for 200 colorimetric or fluorometric tests

Catalog Number: CS0001-1KT

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Category

BCA Protein Assay Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline.

The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H₂O₂ specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).

The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).
Application
Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
Enables evaluation of drug effects on phospholipids metabolism

Suitable for the detection of phospholipids in biological samples
Features and Benefits
Our economical Phospholipid quantification assay kit also saves your time and efforts:

Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples
User friendly: No need to weigh or calculate needed amounts of reagents on your own
Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
Quick instruction bench card - to assure your experimental success
More experiments in one kit - contains sufficient reagents for 200 tests.
Suitable for both colorimetric and fluorometric detection
Detection time: only 30 minutes

Disclaimer
Supply conditions do not apply to the regions and states of Brazil: North, Northeast, Mato Grosso do Sul, Mato Grosso, and Rio Grande do Sul.

Specifications

General

NACRES	NA.84
UNSPSC Code	12352202
usage	sufficient for 200 colorimetric or fluorometric tests
shipped in	dry ice
storage temp.	−20°C

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