Merck, CYTOCOX1-1KT, Cytochrome c Oxidase Assay Kit

Name: Cytochrome c Oxidase Assay Kit
Brand: Merck
SKU: CYTOCOX1-1KT

Size:1 kit, Cytochrome c Oxidase Activity Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

Catalog Number: CYTOCOX1-1KT

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Category

Membrane Protein Extraction Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Biochem/physiol Actions
Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Features and Benefits

Simple, optimized protocol - Obtain reproducible results without special training needs
Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
Use to analyze the intactness of mitochondrial membranes
Simple colorimetric measurement of solution

Specifications

General

NACRES	NA.84
UNSPSC Code	12161503
usage	sufficient for 100 tests
shipped in	dry ice
storage temp.	−20°C
Quality Level	200
Gene Information	human ... COX1(4512)

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