Merck, GF414-MG, HumanKine® HGF, Human Recombinant Xeno-Free

General description
Hepatocyte Growth Factor (HGF), also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotrophic growth factor which is produced by mesodermally derived cells such as endothelial cells, Kupffer cells/macrophages, and hepatic fat-storing cells. HGF/SF is a potent effector of growth, movement, differentiation and invasiveness of epithelial and cancer cells. It can induce epithelial-mesenchymal conversion of epithelial cells in culture, support epithelial branching duct formation in the developing lung and elicit a potent organotrophic function for regeneration of liver, kidney and lung through epithelial-stromal interactions.
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Physical form
Product is presented in PBS and filtered through a 0.2 micron filter before lyophilization.
Preparation Note
HumanKine HGF is manufactured using all non-animal reagents and is considered Xeno-Free
Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
Analysis Note
Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5. ED50 is typically 10-50 ng/ml.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial