Merck, GF439-MG, HumanKine® TGF beta 1, Human Recombinant Xeno-Free

General description
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
Transforming growth factor beta (TGF-B) is a multifunctional cytokine that controls proliferation, differentiation, and other functions in many cell types. TGF-B acts synergistically with TGF-A in inducing transformation. It also acts as a negative autocrine growth factor. TGF-B regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodeling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Physical form
Product is presented in 50mM NaOAc pH 4.5 and filtered through a 0.2 micron filter before lyophilization.
Preparation Note
HumanKine TGF beta1 is manufactured using all non-animal reagents and is considered Xeno-Free
Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
Analysis Note
Bioactivity assay: Determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). ED50 is typically 0.1-0.5 ng/ml.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial