Description
General description
KAPA HotStart® Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA from mouse tissue in less than 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA HotStart Mouse Genotyping Kit is designed for rapid extraction and amplification of DNA from mouse tissue.
Application
KAPA HotStart® Mouse Genotyping Kit has been used for:
extraction and amplification from mouse tail, ear, and toe tissue
extraction and amplification of DNA from other animal tissue
Features and Benefits
Increased throughput, turnaround time and reliability
PCR-ready DNA generated in as little as 15 minutes.
Minimal handling, thereby reducing the risk of sample loss or contamination.
Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format.
Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents.
Outperforms crude extraction methods and commercial kits
Increased turnaround times
Increased specificity
Decreased cost
Quick Notes:
Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes.
Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0–8.5), and use 1 μL of DNA per 25 μL reaction.
Extracts are stable at -20°C for >6 months.
KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl₂ (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
Use 15 sec annealing time, with 15 sec extension time for amplicons
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).
Analysis Note
All components contained with KAPA HotStart Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo and endonuclease activities and meet strict requirements with respect to DNA contamination.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
