Merck, L040082-45L, Cap A

Name: Cap A
Brand: Merck
SKU: L040082-45L

Size:45 L

Catalog Number: L040082-45L

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Category

Reagent Bottles with Cap

Pack Size45 L

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Preparation Note
Acetic Anhydride / Tetrahydrofurane 9.1/90.9 (v/v)
Other Notes
Cap A solution, containing acetic anhydride and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Specifications

General

NACRES	NA.21
UNSPSC Code	12352200
type	for DNA synthesis
product line	Proligo Reagents
Quality Level	200

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