Merck, L3037-1ML, Escort™ III Transfection Reagent

General description
Escort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.
Application
Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)
Biochem/physiol Actions
A stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
Features and Benefits
Suitable for stable and transient transfection
Optimized for a wide variety of primary cells
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for PC-12 cells

Other Notes
Escort™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ III is confidential.
Legal Information
Escort is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Do not freeze.