Merck, L3287-1ML, Escort™ IV Transfection Reagent

General description
Escort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.
Application
Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide.
Biochem/physiol Actions
A stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
Features and Benefits
Suitable for stable and transient transfection
Optimized for a wide variety of cell lines
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for Sf9, Sf21 and S2 insect cells

Other Notes
Escort™ IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ IV is confidential.
Legal Information
Escort is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Do not freeze.