Merck, LGC603002-1KIT, E. cloni EXPRESS BL21(DE3) Electrocompetent Cells (DUOs)

Name: E. cloni EXPRESS BL21(DE3) Electrocompetent Cells (DUOs)
Brand: Merck
SKU: LGC603002-1KIT

Size:12 x 50 μL, Competent Cells for protein expression, DNA cloning, DNA transformation, Electrocompetent E.Coli, Electrocompetent cells, Electroporation, pkg of 24 reactions (12 x 50 µL), Cfu/µg ≥ 5 x 10⁹

Catalog Number: LGC603002-1KIT

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Category

Cell-Free Protein Expression Kits

Pack Size1 Kit

TagsLabware Labware Partners LGC Biosearch™ Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
E. cloni EXPRESS BL21(DE3) Electrocompetent Cells offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.
Application
Cloning, routine protein expression
Features and Benefits
Routine protein expression
High transformation efficiency
Short protein expression protocol time

Transformation efficiency: ≥ 5 x 109 (cfu/µg)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:

E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells

pUC 19 Trasformation Control

Recovery Media

Specifications

General

UNSPSC Code	41106202
NACRES	NB.43
biological source	Escherichia coli
manufacturer/tradename	Lucigen 60300-2
packaging	pkg of 24 reactions (12 x 50 µL)
growth mode	growth or suspension
technique(s)	microbiological culture: suitable
cell transformation	competent cell type: electrocompetenttransformation efficiency: 5×109 cfu/μg
shipped in	dry ice
storage temp.	-65 to -96°C

Ordering

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