Description
General description
m-ColiBlue24® broth is recommended for simultaneous detection of total coliform bacteria and Escherichia coli (E. coli) in water samples within 24 hours. An enzymatic indicator in the medium causes non-fecal total coliform colonies grown on the m-ColiBlue24® medium to be red, while the E. coli (fecal coliform) colonies are blue. The selectivity of the enzymatic indicator eliminates the need for confirmation. The low false positive and false negative rates allow for the detection of at least 95% of all E. coli. m-ColiBlue24® Broth enhances the growth rate of coliform bacteria. Special inhibitors in the medium efficiently minimize the growth of non-coliform bacteria.
Application
m-ColiBlue24® broth is used for monitoring drinking water, wastewater, and other types of water using a 24 hour incubation period. It can also be used to detect coliforms in other types of water—bottled, surface, ground, well, recreational (fresh or marine), as well as process water for ultrapure chemical processing.
Features and Benefits
Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.
EPA Approved
Incubation Temperature: 35 °C ± 0.5
Incubation Time: 24 hrs
Analysis Note
E.coli forms blue colonies. Other coliforms will show as red colonies. All other colors are non-coliform bacteria.
QC organisms
E. cloacae (ATCC 23355)
E. coli (ATCC 25922)
K. pneumoniae (ATCC 13883)
P. aeruginosa (ATCC 27853)
Target Microorganisms: Total coliforms and E. Coli
Legal Information
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
m-ColiBlue24 is a registered trademark of Hach Company
