Merck, M2003-1G, MTT Formazan

Name: MTT Formazan
Brand: Merck
SKU: M2003-1G

Size:1 g, 1-(4, 5-Dimethylthiazol-2-yl)-3, 5-diphenylformazan, Thiazolyl blue formazan, powder

Catalog Number: M2003-1G

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Category

Special Stains

Pack Size1 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
MTT Formazan has been used to measure cell viability.
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24896591
UNSPSC Code	12171500
MDL number	MFCD00080731
InChI	1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
SMILES string	Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI key	GIFKVQOPAJDJLO-SZVLODPRSA-N
assay	≥97% (TLC)
form	powder
color	purple
solubility	acetone: 1 mg/mL
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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