Merck, MAB1285Z-M, Anti HLA-B27, Clone.ABC.M3, Purified (1mg) KC

General description
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.
Application
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Cytotoxicity testing 1:25.
Biochem/physiol Actions
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Cell reactivity:

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:



Subjects Fluorescence Intensity

Heterozygous HLA-B27+ Strong

Homozygous HLA-B7+ Strong

Heterozygous HLA-B7+ Faint

Non B27, Non B7 Negative
Physical form
The antibody is supplied in phosphate-buffered saline, pH 7.4.
Preparation Note
Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Disclaimer
This product is intended for use by IVD manufacturers.
Contact customer service for assistance: [email protected]
Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.