Merck, MAB8566, Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5

Application
Detect Poliovirus Blend using this Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1 & 613-F1-B5-E5 validated for use in IF.
IFA at 1:100-1:400.

Dilute with buffer pH 7.5-8.0 to desired working volume.

Final working dilutions must be determined by end user.

SUGGESTED IFA PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μl diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μl diluted labeled antibody.

5. Repeat steps 2 and 3.

6. Mount with buffered glycerol.
Biochem/physiol Actions
Reacts with the Poliovirus 1, 2 and 3.
Preparation Note
Maintain at -20°C in convenient undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany