General descriptionEnrofloxacin is a fluoroquinolone antibiotic used for the treatment of pets and domestic animals and in fish farming. Minimum acceptable limits have been set for enrofloxacin residues in food samples. The enrofloxacin extraction kit enables the simultaneous separation of enrofloxacin from tissue, and the defatting of the sample during extraction in an accurate and quick manner.
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ApplicationEnrofloxacin concentrations (ng/g) can be derived from biological specimens. Can be used for any biological food sample-shrimp, meat, liver, crab fish, etc. This kit enables the extraction of enrofloxacin for quantitation by ELISA.
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for a video demonstration of how the kit worksThis kit is suitable for the extraction of enroflaxacin from a variety of samples such as biological tissue and food samples.
Biochem/physiol ActionsThe Enrofloxacin Extraction Kit enables the simultaneous separation of enrofloxacin from tissue, and the defatting of the sample during extraction in an accurate and quick manner. The streamlined process bypasses the centrifugation, vortexing, pipetting and solvent addition steps associated with extracting and defatting the sample. The extracted enrofloxacin can then be detected and quantified by enzyme-linked immunosorbent assay (ELISA) followed by spectrophotometric analysis.
Enrofloxacin is typically extracted using a single solvent system containing 80% methanol in buffer. In the standard procedure, methanol containing the enrofloxacin is separated from tissue by centrifugation for 10 minutes followed by manual pipetting. The extract is then dried and reconstituted into 1 mL of 8% methanol in sample dilution buffer. The extract is subsequently defatted by adding 1 mL hexane and centrifuging. A 50 μL aliquot is taken from the bottom layer containing the defatted enrofloxacin extract and used for analysis by ELISA and spectrophotometry.
The Enrofloxacin Extraction Kit shortcuts the extraction process by eliminating the need for centrifugation, manual pipetting, and post-extraction defatting with hexane or another lipophilic solvent. After adding 80% methanol to the sample, it can be mixed manually for 15 minutes or with sonication for 5 minutes and poured through the syringe containing a filter. The eluted solution containing the enrofloxacin extract is then reconstituted in 8% methanol and a 50 μL aliquot is used for analysis by ELISA and spectrophotometry.
Features and BenefitsThe streamlined process bypasses the centrifugation, vortexing, pipetting and solvent addition steps associated with extracting and defatting the sample. The extracted enrofloxacin can then be detected and quantified by enzyme-linked immunosorbent assay (ELISA) followed by spectrophotometric analysis.
