Merck, MAK492-1KT, Fumarate Assay Kit

Name: Fumarate Assay Kit
Brand: Merck
SKU: MAK492-1KT

Size:1 kit, sufficient for 100 colorimetric tests

Catalog Number: MAK492-1KT

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Category

Diagnostic ELISA Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Fumarate, or fumaric acid, is one of the key components in the TCA cycle and is used by cells to form ATP. Human skin, when exposed to sunlight, will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industry. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs Cycle function, a defect in the enzyme fumarase, or mitochondrial function.
Application
The kit is suitable for the determination of fumarate in food, beverage, and other biological samples (e.g. cell lysate, tissue homogenate, and serum).
Biochem/physiol Actions
The Fumarate Assay Kit is based on the fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH, which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm, is proportional to the fumarate concentration in the sample.
Features and Benefits

Linear detection range 0.005 to 2 mM fumarate.
Compatible with high-throughput handling systems.

Specifications

General

UNSPSC Code	12352200
NACRES	NA.84
application(s)	food and beverages
detection method	colorimetric
shipped in	wet ice
storage temp.	−20°C
Quality Level	100

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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