Merck, MAK512-1KT, Malate Dehydrogenase Assay Kit

Name: Malate Dehydrogenase Assay Kit
Brand: Merck
SKU: MAK512-1KT

Size:1 kit, sufficient for 100 colorimetric tests

Catalog Number: MAK512-1KT

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Category

Diagnostic ELISA Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.
Application
The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)
Biochem/physiol Actions
The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Features and Benefits

Linear detection range: 0.5 to 65 U/L
Compatible with high-throughput handling systems.

Specifications

General

NACRES	NA.84
UNSPSC Code	12352204
detection method	colorimetric
relevant disease(s)	Alzheimer′s disease; neurological disorders; neurodegenerative disorders
shipped in	wet ice
storage temp.	−20°C
Quality Level	100

Ordering

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