Merck, MAK525-1KT, Acid Phosphatase Fluorometric Assay Kit

General description
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.
Application
The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).
Biochem/physiol Actions
The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Features and Benefits

• Linear detection range: 0.008 to 10 U/L .
• Compatible with high-throughput handling systems.