Merck, N7404, Niacin assay medium

Application
Recommended for the microbiological assay of Niacin or Niacinamide.
Preparation Note
Suspend 7.51 g of Niacin Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For the assay, dispense 5 ml of medium per assay tube (containing increasing amounts of standard or unknown) and make up the total volume to 10 ml per tube with distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool immediately.
Other Notes
Ingredients (g/L)
Casein acid hydrolysate vitamin free, 12.00
Dextrose, 40.00
Sodium acetate, 20.00
L-Cysteine, 0.40
DL-Tryptophan, 0.20
Adenine sulphate, 0.02
Guanine hydrochloride, 0.02
Uracil, 0.02
Thiamine hydrochloride, 0.0002
Calcium pantothenate, 0.0002
Pyridoxine hydrochloride, 0.0004
Riboflavin, 0.0004
p-Aminobenzoic acid, 0.0002
Biotin, 0.00008
Dipotassium phosphate, 1.00
Monopotassium phosphate, 1.00
Magnesium sulfate, 0.40
Sodium chloride, 0.02
Ferrous sulfate, 0.02
Manganese sulfate, 0.02
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany