Merck, NA29-100UG, Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4)

General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-2 mouse myeloma cells. Recognizes the ~16 kDa p16 protein.
Recognizes the ~16 kDa p16 protein in Saos-2 cells and bladder tissue. Sold under license of U.S. Patent 6,482,929. Sold under license of U.S. Patent 5,843,756, 6,090,578, and corresponding patents.
This Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4) is validated for use in Immunoblotting for the detection of p16 (Ab-1).
Immunogen
Human
recombinant, human p16
Application
Immunoblotting (0.5-5 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin pH 7.5.
Analysis Note
Negative Control
U2OS cells
Positive Control
Saos-2 cells or bladder tissue
Other Notes
Does not cross-react with the closely related p15 protein. Antibody should be titrated for optimal results in individual systems.
Koh, J., et al. 1995. Nature375, 506.
Lukas, J., et al. 1995. Nature375, 503.
Cheng, J.Q., et al. 1994. Cancer Res.54, 5547.
Jen, J., et al. 1994. Cancer Res.54, 6353.
Kamb, A., et al. 1994 Science264, 436.
Nobori, T., Miura, K., et al. 1994. Nature368, 753.
Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 11045.
Serrano, M., et al. 1993. Nature366 704.
Legal Information
Sold under license of U.S. Patents 5,843,756, 6,090,578 and corresponding patents.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)