Merck, PPB021-20PAK, SSPE Buffer

Application
Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.
Features and Benefits
Ready-to-use buffer
No measuring and pH adjusting needed
Save time and minimize effort
Biological tests: free of DNase, RNase, protease, and nickase
Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
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Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC