Description
General description
Aberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included.
Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
*p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146.
Application
Principles of the Technique
Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
