Description
General description
Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Polymyxin B is a cationic lipopeptide antibiotic that binds specifically to Gram-negative bacteria. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.
Dansyl labeled Polymyxin B can be utilized for fluorescent microbial imaging in the fields of Cell Biology and Biochemical research.
Application
DSL-PMB may have been used:as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
in research and to develop new active derivatives of Polymyxin against multi-drug resistance Gram-negative infections
to measure LPS binding affinity by fluorometric displacement assay
Fluorescent antibiotics can be used for many applications including:Antimicrobial resistance research.
Bacterial visualization and imaging.
Parent antibiotic mode of action research and new antibiotic discovery.
Toxicity studies.
Research of bacterial infections and tracking its uptake in vivo.
Biochem/physiol Actions
DSL-PMB′s mode of action is in accordance with Polymyxin B activity. However, MIC assays comparing DSL-PMB, and parent Polymyxin B showed a two to three-fold decrease in bacterial activity. Polymyxin B interacts with anionic lipopolysaccharide (LPS) molecules which are located in Gram-negative bacterial outer membrane. This interaction interferes with the outer membrane′s normal function, causing leakage and enhanced uptake of the antibiotic. The resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label Gram-negative bacteria and examine intracellular localization and penetration of Polymyxins in Gram-negative bacteria.
Features and Benefits
High-quality antibiotic suitable for multiple research applications
Ideal for Cell Biology, Metabolomics, and Biochemical research.
Analysis Note
Dansyl Labeled Polymyxin B Ready Made Solution is light sensitive.
It is recommended to avoid freeze-thaw cycles of PMB-DSL Ready Made Solution.
Dansyl Labeled Polymyxin B Ready Made Solution (1.5 mg/mL) can be diluted 1:50 in PBSX1 (Sigma#D8537) to achieve 30 μg/mL final concentration for staining. The above concentration of DSL-PMB was used for E. coli staining see image.
Fluorescence Microscopy application: Dansyl Labeled Polymyxin B Ready Made Solution excitation (Ex) wavelength is 330-340nm resulting in emission (Em) range of 540-600nm (λmax=570nm).
Other Notes
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