Merck, SBR00030-1KT, Short Chain Fatty Acid Kit

Name: Short Chain Fatty Acid Kit
Brand: Merck
SKU: SBR00030-1KT

Size:1 kit, Sodium acetate, Sodium propionate, and Sodium butyrate

Catalog Number: SBR00030-1KT

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Category

Lipid Panel Reagents

TagsChemistry & Biochemicals Biochemicals Lipids

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Short chain fatty acids (SCFAs) are saturated fatty acids with less than 6 carbons. SCFAs are produced in the gut by microbial fermentation of dietary fibers.(1) The most important and researched SCFAs are Acetate (Ac), propionate (Pr) and butyrate (Bu).(2) SCFAs are predominantly found in the colon and cross the intestinal epithelium to the blood stream. SCFAs were found to be beneficial and effective in prevention of obesity, Non-alcoholic fatty liver disease and insulin resistance.(3,4)
Application
SCFAs can serve in various applications including in vitro and in vivo administration to study the effect on immune system activation(5), protect epithelial cells from LPS penetration by tight junction formation induction(6), effect insulin secretion(7) and more. In these studies, SCFAs were administrated in vitro at the following concentration range Acetate 1-10mM, propionate and butyrate 0.1-1mM and in vivo Acetate, 70 mM; propionate, 30 mM; butyrate, 20 mM.
Short-chain fatty acids (SCFAs) have diverse roles in various metabolic processes, and their measurement through metabolomics can provide valuable insights into several applications.
Biochem/physiol Actions
Role
Regulate metabolism in the gut
Indicators of gut health and inflammation
Associated with gastrointestinal diseases such as IBS and other conditions such as obesity, colonic carcinogenesis, insulin sensitivity and ischemic stroke

Features and Benefits
High-quality chemical, suitable for mulitple research applications
Commonly employed in Metabolomics and Biochemical studies

Other Notes
For additional information on our range of Biochemicals, please complete this form.

Specifications

General

NACRES	NA.25
UNSPSC Code	12352211
packaging	pkg of 1 kit
lipid type	saturated FAs
storage temp.	−20°C
Quality Level	100

FAQs

Question: Which compounds are included in the kit?

Answer:Sodium acetate, sodium propionate and sodium butyrate solutions, plus a mixed solution of the three compounds.

Question: What are the individual solution concentrations and volumes?

Answer:Sodium acetate is 1 M in 100 mL; sodium propionate and sodium butyrate are each 0.5 M in 100 mL; the mixed reference volume is 1 mL.

Question: What storage temperature is specified?

Answer:The kit technical bulletin specifies storage at −20 °C.

Application Note

The Short Chain Fatty Acids Kit supplies prepared aqueous sodium acetate, sodium propionate and sodium butyrate solutions for experimental studies of short-chain fatty acid effects. The components comprise 100 mL of 1 M sodium acetate, 100 mL of 0.5 M sodium propionate, 100 mL of 0.5 M sodium butyrate and a 1 mL mixture containing these analytes at a nominal 0.1 M formulation. The kit supports controlled dosing in cell-based studies and provides a mixed reference preparation for method development in chromatographic analysis. Record final concentrations and relevant medium controls for each study; biological effects can differ by compound, matrix and exposure conditions. Store the prepared reagents at −20 °C and follow the kit handling instructions.

Ordering

Notice

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