General descriptionJK1 is an immortalized CD34+SMA+ cell line derived from spontaneous transformation of C57/BL6 mouse testicular stromal cells. JK1 cells are able to support the long-term culture of a variety of primitive cells including multi-potent adult spermatogonial-derived stem cells, pluripotent murine embyronic stem cells and embyronic germ cells derived from primordial germ cells. Adult spermatogonial stem and progenitor cells (SPCs) cultured on JK1 feeder cells express key features of germ line stem cells including expression of PLZF, DAZL, and GCNA1. JK1 feeder cells has maintained its capacity for promoting stem cell self-renewal even after serial passaging for over one year.
ApplicationImmortalized Mouse Feeder Cell Line
Research Category
Stem Cell Research
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Biochem/physiol ActionsFibroblast Cells
Preparation NoteEmbryoMax JK1 Murine Testicular Stromal Feeder Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.
Legal InformationEmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
