General descriptionThe SMA-560 cell line is a well-established glioma model derived from a spontaneous murine astrocytoma (2,3). SMA560 cells are highly reflective of differentiated anaplastic astrocytoma, having high expression of the differentiated astrocyte marker glial fibrillary acid protein (GFAP) and the astrocyte marker glutamine synthetase and low expression of S-100 proteins (4,5). The SMA560 cell line maintains robust tumorigenic behavior after serial passaging (2). Uniquely among established murine glioma models, SMA560 cells express the immunosuppressive protein transforming growth factor β (TGF-β) (6), making this cell line of great value for investigation of targeted cancer immunotherapies.
Source:
SMA-560 cell line was derived from a spontaneous astrocytoma passaged in syngenic VM/Dk mice (2).
Gliomas are a rare and aggressive cancer with an extremely low survival rate and high resistance to treatment (1). Glioma models that recapitulate the multiple features of the disease are important for understanding of mechanisms of glioma malignancy and development of effective therapies, especially those that address tumor-induced immunosuppression and resistance.
References:
1. N Engl J Med 2005; 352(10): 987-996.
2. Acta Neuropathol 1980; 51(1): 53-64.
3. Neurosci Lett 1982; 34(3): 315-320.
4. J Neurol Sci 1983; 62(1-3): 115-139.
5. J Neurol Neurosurg Psychiatry 1986; 49(12): 1361-1366.
6. Neurosurgery 1997; 41(6): 1365-1372.
ApplicationSMA-560 cell line is a well-established glioma model derived from a spontaneous murine astrocytoma.
Biochem/physiol ActionsCancer Cells
Packaging≥1X10⁶ cells/vial
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis NoteEach vial contains ≥ 1X10⁶ viable cells.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
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