General descriptionCystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a chloride and bicarbonate ion channel (1). Among the approximately 150 known disease-causing mutations in CFTR, deletion of the phenylalanine-508 codon (ΔF508) is the most common and results in protein destabilization, misfolding and ultimately degradation (2). Although the most pronounced lung disease symptoms of CF, including chronic bacterial infection and purulent airway obstruction, are highly associated with neutrophils, the lack of cellular models for CF neutrophils has hindered progress in understanding the significance of these cells for CF pathogenesis (3).The HL-60 DeltaF508-CF human promyelocytic cell line is a derivative of the HL-60 promyelocytic leukemia cell line that has been edited via CRISPR/Cas9 technology for homozygous deletion of CFTR F508 (3). Differentiation of HL-60 DeltaF508-CF cells into neutrophils occurs upon treatment with DMSO. Differentiated HL-60 DeltaF508-CF cells demonstrate normal levels of CD11b staining but substantially reduced CFTR surface expression compared to differentiated HL-60 control cells, in addition to compromised bactericidal activity (3). Source:The HL-60 DeltaF508-CF human promyelocytic cell line was derived from HL-60 promyelocytic leukemia cells transfected with a hCFTR-Cas9-eGFP plasmid. Single-cell clones were sorted for eGFP expression and screened for homozygous deletion of CFTR F508 via nuclease digestion and DNA sequencing (3).
Biochem/physiol ActionsHuman, Cancer Cells
Packaging≥1X10⁶ cells/vial
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerRESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
