General description
Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.
LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.
Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
This product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see SCC064.
Hepatic stellate cells are an essential cell phenotype responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for the development of therapeutics.
The LX-2 cell line was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early-passage cells in low serum media conditions.
Features and Benefits
LX-2 cells retain key features of human hepatic stellate cells including cytokine signaling, retinoid metabolism, and fibrogenesis. **SCCAR10064 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.
Preparation Note
LX-2 cells should be stored in liquid nitrogen.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
