Description
General description
Endothelial Cell Characterization Kit
Antibodies for the immunocytochemical characterization of endothelial cells.
Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon′s endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.
Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:
* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL
* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL
The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:
* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml
Application
Research Category
Stem Cell Research
Preparation Note
Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).
Other Notes
50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)
50 μg of CD31 monoclonal (Part No. 2003788)
10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)
50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)
50 μg of ICAM-1 monoclonal (Part No. 2003785)
100 μg of Mouse control IgG (Part No. PP54-100UG)
100 μg of Rabbit control IgG (Part No. PP64-100UG)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
