Description
General description
The ENStem-A Human Neural progenitor Expansion Kit contains ENStem-A Human Neural Progenitors and optimized Expansion medium. ENStem-A Human Neural Progenitor Cells are derived from NIH approved H9 human embryonic stem cells (hESCs). Using a proprietary method developed by Aruna Biomedical, these hESC derived neural progenitors proliferate as an adherent cell monolayer and can readily differentiate into different neuronal subtypes. Detailed spectral analyses indicate ENStem-A cells retained a normal diploid karyotype in culture after ten passages. ENStem-ATM human neural progenitor cells may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-A Human Neural Progenitor Cells.
Preparation Note
EnStem Human Neural Progenitor Cells: When stored at the recommended storage conditions (liquid nitrogen), ENStem-A cells are stable up to five years. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages. ENStem-A Human Neural Expansion media ENStem-A Neural Expansion Medium should be stored at -20C until ready to use. Upon thawing, the expansion medium should be stored at 2-8C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
Analysis Note
Each lot of ENStem-A Human Neural Progenitor Cells has been validated for high levels of expression of Nestin and Sox 2, and low level expression of Oct-4. The ability of ENStem-A cells to differentiate into multiple neuronal phenotypes and maintain a normal karyotype after multiple passages has been verified (please refer to datasheet figures). The cells have been confirmed to be negative for mycoplasma.
Other Notes
ENSTem-A Human Neural Progenitors, 1 x 10⁶ cells ENStem-A Neural Expansion Media, SCM004, 500 mls Basic FGF, lyophilized, 10 ug
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
