Description
General description
EMD Millipore′s Human Dopaminergic Neurogenesis Kit (Cat No. SCR135) contains the necessary growth factors and complete differentiation media (Cat No. SCM110 and SCM111) needed to differentiate human ES/iPS cells into dopaminergic neurons using a 21 day differentiation protocol. The protocol has been validated with 4 human ES and iPS lines for efficient dopaminergic neuronal differentiation.
H1: 30% TH positive neurons
H9: 50-70% TH positive neurons.
Two Human iPS cell lines: ~ 50% TH positive neurons.
The Dopaminergic Differentiation Growth Factor Sampler (SCR128) contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydroxylase, to aid in quantifying the percentage yields from differentiation experiments. Included in the kit are the following:
2 vials of human recombinant Sonic Hedgehog (Shh) (5 ug/vial)
1 vial of human recombinant FGF-8 (5 ug/vial)
1 vial of human recombinant BDNF (2 ug/vial)
1 vial of human recombinant GDNF (2 ug/vial)
1 vial of human recombinant TGF-b-III (2 ug/vial)
1 vial of anti-tyrosine hydroxylase, rabbit polyclonal (20 uL)
The Human ES/iPS Neural Induction Medium (Cat. No. SCM110) and Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111) are robust and user-friendly media that contain the necessary media and reagents for the rapid production of expandable neural progenitor cells and terminally differentiated neurons from pluripotent human ES and iPS cells using small molecule inducers.
Preparation Note
All components within SCR135 (Cat. No. SCM110, SCM111 and SCR128) should be stored at -20C upon receiving.
Other Notes
1) Human ES/iPS Neural Induction Medium: (Catalog No. SCM110). Store at -20C. 2) Human ES/iPS Neuronal Differentiation Medium: (Catalog No. SCM111). Store at -20C. 3) Dopaminergic Differentiation Growth Factor Sampler (Cat. No. SCR128). Store at -20C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
