Merck, SCT066, BioTracker Tau filaments pFTAA Live Cell Dye

Name: BioTracker Tau filaments pFTAA Live Cell Dye
Brand: Merck
SKU: SCT066

Size:1 mg, Live cell imaging probe

Catalog Number: SCT066

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Category

Fluorescent Dyes

Pack Size1 mg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Protein misfolding diseases are characterized by deposition of protein aggregates. Accumulation of protein deposits, so-called amyloid, is the histopathological hallmark of several devastating diseases, and the development of sensitive optical ligands to detect and characterize these disease-associated structures is of great interest.

The BioTracker Tau filaments pFTAA live cell dye is a novel fluorescent probe that can bind to and discriminate between amyloid-b (A?) plaques and tau neurofibrillary tangles (NFTs) in Alzheimer′s disease (AD)-like pathology in transgenic mouse brain tissue sections.

Spectral Properties:
Excitation: 405 nm
Emissions: 550 nm

References:
Klingstedt T et al. The Structural Basis for Optimal Performance of Oligothiophene-Based Fluorescent Amyloid Ligands: Conformational Flexibility is Essential for Spectral Assignment of a Diversity of Protein Aggregates. Chem Eur J. 2013, 19, 10179-10192.
Application
Application: Live cell fluorescent imaging
Live cell imaging dye for detection of disease-associated protein aggregates such as Amyloid protein deposits.
Analysis Note
Purity: ≥98% confirmed by HNMR, LC-MS and HPLC and elemental analysis

Molar Mass: 704.65 g/mol
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Specifications

General

NACRES	NA.32
UNSPSC Code	12171501
technique(s)	cell based assay: suitable

FAQs

Question: What type of marker is pFTAA?

Answer:A fluorescent oligothiophene ligand used to visualize selected amyloid and protein-aggregate structures, not a tau-specific antibody.

Question: Which approximate wavelengths are relevant?

Answer:Excitation near 405 nm and emission near 550 nm are reported starting points for imaging setup.

Question: Can fluorescent signal alone confirm a tau-specific diagnosis?

Answer:No. Aggregate binding and signal interpretation require appropriate controls and independent target-specific validation.

Application Note

Millipore BioTracker pFTAA is a conjugated oligothiophene fluorescent probe used in research into misfolded protein aggregates, including tau-associated neurofibrillary structures and amyloid-related deposits. Its fluorescence response to aggregate-associated molecular environments supports imaging and comparison of selected amyloid structures in research specimens and compatible live-cell workflows. Characteristic reported excitation and emission wavelengths are approximately 405 nm and 550 nm, but spectral response, background and optimum acquisition settings depend on the sample and imaging system. The dye is not a target-specific monoclonal antibody and cannot independently establish tau pathology or distinguish all amyloid species in every biological matrix. Include untreated and appropriate positive/negative controls, optimize staining concentrations experimentally and assess photobleaching and nonspecific background. Suitable workflows include fluorescence microscopy of research models investigating protein aggregation and conformational disease biology. Maintain dye handling conditions to avoid moisture, light and solvent-related changes, and follow the product safety and storage guidance for the supplied batch.

Ordering

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