Description
General description
BioTracker™ Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator is a cell-membrane-permeable, perylene-based dye. It specifically localizes to mitochondria via introduction of the triphenylphosphonium moiety. SCT261 is used to monitor lipid peroxidation in ferroptosis research.With excitation and emission wavelengths of 452 nm and 470 nm respectively, this probe can be applied for lipophilic peroxide imaging in living cells.
This product is a cell-membrane-permeable, perylene-based probe for use in live cell cultures. The compound specifically localizes in mitochondria due to the triphenylphosphonium moiety. It is therefore optimal for monitoring lipid peroxidation in ferroptosis.IMPORTANT APPLICATION NOTES: Prepare 0.1 mM stock solution by dissolving the 5ug of SCT261 in 50 uL DMSO. Lyophilized probe may be difficult to see, confirm with yellow color of working solution. Protect solution from light and use within the same day. Dilute with Hanks′ HEPES buffer to prepare 0.1 to 0.5 uM working solution.SPECTRAL PROPERTIESλex: 452 nmλem: 470 nmSuitable for use with FITC/GFP laser or filter
Features and Benefits
This probe is designed for the detection of lipid peroxidation in live cells by fluorescent microscopy or flow cytometry.
Preparation Note
Store BioTracker™ Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator at 2 - 8 °C, protected from light.
Other Notes
Live cell probe for fluorescent detection of lipid peroxidation in mitochondria by fluorescence microscopy or flow cytometry. SCT261 is an essential tool for ferroptosis research in live cell models.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
